DNA or
De-oxy Ribonucleic acid can be looked at as an integral part of the human body
or as an interesting piece of evidence. The beautiful color of your eyes, the
shining hair color, bone density and much more is due to the DNA. Each cell in
our body consists of a DNA strand and some of the DNA formations are in a
particular code like AATTGCCTTTTAAAAA. These are called coding DNA's and this
same code is passed over from the parents to the offspring through sperm and
egg. vip pcr home abu dhabi This is also the type of DNA that forensic experts use for
various testing purposes.
In the last
decade or so, forensic science has advanced at a faster rate and DNA is used
not only for finding criminals but also solving cases, which have been long
buried in a heap of files. DNA testing for forensic evidence can lead to the
following:
1. It can assist in
solving crimes like murders, rape etc.
2. It can assist in
archeological discoveries like determining the origin, history and physical or
health conditions of the mummies of Egypt
3. Testing DNA can
also determine the family history
4. DNA testing can
provide information regarding the parents, the mother or father of a child.
There are
millions of possibilities and results that can be arrived through DNA testing
but the truth is that forensic experts and scientists have only been able to
uncover a small but significant part of the mystery of DNA.
DNA testing
for forensic evidence can be broadly termed in two main groups. The first type
is called the RFLP testing and second is PCR based testing. The RFLP DNA
testing process can only be done when a large amount of DNA is available. The
DNA evidence normally derived from the crime-scene is normally present in
smaller amounts and can also be too old, which makes it unsuitable for RFLP
testing. When conditions are warm or moist then it may cause the DNA
degradation, which will again make RFLP DNA testing almost impossible.
One the
other hand, PCR-based forensic testing will require less DNA as compared to
RFLP testing. Even if the DNA is in a partially degraded condition, PCR testing
DNA is possible. Most forensic experts have to keep in mind that even DNA
samples for PCR testing has limitations as far as DNA degradation and sample
size is concerned.
One aspect
that the PCR-based test is extremely sensitive to is contamination of DNA found
at the crime scene. Contaminations can even happen in the test laboratory.
Basically, contaminants in DNA can influence the PCR results and lead to the
failure of the test. The RFLP tests are preferred as compared to PCR because
the latter is prone to error.
DNA testing
using the RFLP method requires forensic vip pcr home abu dhabi experts to cut the DNA using a restriction enzyme
before they can use it to find information. This restriction enzyme is
important because it can recognize a particular sequence of the DNA like the
AATT sequence. The commonly used restriction enzyme is Hae III and the choice
of enzyme depends purely on the forensic experts.
Comments
Post a Comment